Effects of intravesicular loading of a Ca 2+ chelator and depolymerization of actin fibers on neurotransmitter release in frog motor nerve terminals.

Ca ²⁺ -induced Ca ²⁺ release (CICR) via type-3 ryanodine receptor enhances neurotransmitter release in frog motor nerve terminals. To test a possible role of synaptic vesicle in CICR, we examined the effects of loading of EGTA, a Ca ²⁺ chelator, into synaptic vesicles and depolymerization of actin fibers. Intravesicular EGTA loading via endocytosis inhibited the ryanodine sensitive enhancement of transmitter release induced by tetanic stimulation and the associated rises in intracellular-free Ca ²⁺ ([Ca ²⁺ ] _i : Ca ²⁺ transients). Latrunculin A, a depolymerizer of actin fibers, enhanced both spontaneous and stimulation-induced transmitter release, but inhibited the enhancement of transmitter release elicited by successive tetanic stimulation. The results suggest a possibility that the activation of CICR from mobilized synaptic vesicles caused the enhancement of neurotransmitter release.
(© 2019 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)