HPLC-MS/MS analysis of ilimaquinone and its application in a pharmacokinetic study in rats.

Authors :: Son H
Noh K
Kang C
Na M
Oh S
Song IS
Kang W
Source :: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2019 Mar 20; Vol. 166, pp. 291-294. Date of Electronic Publication: 2019 Jan 17.
Publication Year :: 2019
Abstract: Ilimaquinone, a metabolite isolated from the marine sponge Hippiospongia metachromia, has antimicrobial, cytotoxic, anti-HIV, anti-inflammatory, and anti-cancer activities. A new quantitative analytical method for determination of ilimaquinone in rat plasma using HPLC-MS/MS was developed and validated. Ascorbic acid was added to ensure the stability of ilimaquinone in plasma. After protein precipitation using acetonitrile plus diclofenac as an internal standard, the analytes were chromatographed on a biphenyl column with a mobile phase of methanol and water (8:2, v/v, including 0.1% formic acid). This method was successfully applied in a pharmacokinetic study of ilimaquinone after oral administration in rats.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)

Subjects :: Administration, Oral
Animals
Chromatography, High Pressure Liquid
Drug Stability
Porifera chemistry
Quinones administration & dosage
Quinones blood
Quinones isolation & purification
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Sesquiterpenes administration & dosage
Sesquiterpenes blood
Sesquiterpenes isolation & purification
Tandem Mass Spectrometry
Quinones pharmacokinetics
Sesquiterpenes pharmacokinetics

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