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MiR-182-5p Knockdown Targeting PTEN Inhibits Cell Proliferation and Invasion of Breast Cancer Cells.

Authors :
Zhao YS
Yang WC
Xin HW
Han JX
Ma SG
Source :
Yonsei medical journal [Yonsei Med J] 2019 Feb; Vol. 60 (2), pp. 148-157.
Publication Year :
2019

Abstract

Purpose: Breast cancer (BC) is one of the most common malignant tumors, affecting a significant number of women worldwide. MicroRNAs (miRNAs) have been reported to play important roles in tumorigenesis. The aim of this study was to determine the roles of miR-182-5p in BC progression.<br />Materials and Methods: The expressions of miR-182-5p and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) were measured in BC tissues and cells by quantitative real-time polymerase chain reaction or Western blot. Cell proliferation and invasion were detected by cell counting kit-8 assay and trans-well assay, respectively. The interaction between miR-182-5p and PTEN was probed by bioinformatics analysis, luciferase activity, and RNA immunoprecipitation. A murine xenograft model was established to investigate the role of miR-182-5p in BC progression in vivo .<br />Results: An abundance of miR-182-5p was noted in BC tissues and cells. High expression of miR-182-5p was associated with poor survival. Abrogation of miR-182-5p inhibited cell proliferation and invasion in BC cells. Interestingly, PTEN was indicated as a target of miR-182-5p, and its restoration reversed miR-182-5p-mediated promotion of proliferation and invasion of BC cells. Moreover, depletion of miR-182-5p suppressed tumor growth via up-regulating PTEN expression in the murine xenograft model.<br />Conclusion: MiR-182-5p exhaustion blocked cell proliferation and invasion by regulating PTEN expression, providing a novel therapeutic avenue for treatment of BC.<br />Competing Interests: The authors have no potential conflicts of interest to disclose.<br /> (© Copyright: Yonsei University College of Medicine 2019.)

Details

Language :
English
ISSN :
1976-2437
Volume :
60
Issue :
2
Database :
MEDLINE
Journal :
Yonsei medical journal
Publication Type :
Academic Journal
Accession number :
30666836
Full Text :
https://doi.org/10.3349/ymj.2019.60.2.148