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Analysis of Tissue-Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1913, pp. 217-222. - Publication Year :
- 2019
-
Abstract
- The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 <superscript>+</superscript> T cells by multi-parametric flow cytometry.
- Subjects :
- Animals
Cell Separation instrumentation
Flow Cytometry instrumentation
Immunologic Memory
Lung immunology
Mice
Single-Cell Analysis instrumentation
Single-Cell Analysis methods
Skin immunology
CD8-Positive T-Lymphocytes immunology
Cell Separation methods
Flow Cytometry methods
Lung cytology
Skin cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1913
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30666610
- Full Text :
- https://doi.org/10.1007/978-1-4939-8979-9_16