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Quantitative analysis of enasidenib in dried blood spots of mouse blood using an increased-sensitivity LC-MS/MS method: Application to a pharmacokinetic study.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2019 Jun; Vol. 33 (6), pp. e4491. Date of Electronic Publication: 2019 Feb 07. - Publication Year :
- 2019
-
Abstract
- A simple, sensitive and rapid assay method has been developed and validated as per regulatory guidelines for the estimation of enasidenib on mouse dried blood spots (DBS) using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The method employs liquid extraction of enasidenib from DBS disks of mouse whole blood followed by chromatographic separation using 0.2% formic acid-acetonitrile (25:75, v/v) at a flow rate of 1.0 mL/min on an Atlantis dC <subscript>18</subscript> column with a total run time of 2.0 min. The MS/MS ion transitions monitored were m/z 474.0 → 267.1 for enasidenib and m/z 309.2 → 251.3 for the internal standard (warfarin). The assay was linear in the range of 1.01-3044 ng/mL. The within-run and between-run precisions were in the range of 3.18-9.06 and 4.66-8.69%, respectively. Stability studies showed that enasidenib was stable on DBS cards for 1 month. This novel method has been applied to analyze the DBS samples of enasidenib obtained from a pharmacokinetic study in mice.<br /> (© 2019 John Wiley & Sons, Ltd.)
- Subjects :
- Aminopyridines chemistry
Animals
Limit of Detection
Linear Models
Male
Mice
Mice, Inbred BALB C
Reproducibility of Results
Triazines chemistry
Aminopyridines blood
Aminopyridines pharmacokinetics
Chromatography, Liquid methods
Dried Blood Spot Testing methods
Tandem Mass Spectrometry methods
Triazines blood
Triazines pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 33
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 30663096
- Full Text :
- https://doi.org/10.1002/bmc.4491