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mRNA Expression Profile of SFKs and Involvement of SFKs in the Regulation of LPS-Induced Erk1/2 Signaling in PBMCs of Active BD Patients.
- Source :
-
Endocrine, metabolic & immune disorders drug targets [Endocr Metab Immune Disord Drug Targets] 2019; Vol. 19 (6), pp. 809-817. - Publication Year :
- 2019
-
Abstract
- Background: Behcet's Disease (BD) is a multisystemic inflammatory disorder affecting large vessels, lungs joints, gastrointestinal and neurological systems. The pathogenesis of BD remains poorly understood. Identifying the key signaling pathway is crucial for a complete understanding of the pathogenesis of BD.<br />Objective: The aim of this study was to determine mRNA expression level of Src family kinases (SFKs) members and their involvement in lipopolysaccharide (LPS)-induced mitogen-activated protein kinases (MAPKs) regulation in peripheral blood mononuclear cells (PBMCs) of active BD patients.<br />Methods: Twenty- five active BD patients and twenty-five healthy controls were included in the study. PBMCs were isolated from total blood by density gradient centrifugation. The mRNA expression levels of SFKs members were measured by real-time quantitative PCR (RT-qPCR). The effect of SFKs activity on LPS-induced activation MAPKs (Erk1/2, p38 and JNK) was examined by Western blot.<br />Results: The mRNA expression levels of Hck, Src, Lyn, Yes and Fyn were found to be slightly decreased in active BD patients compared to the control subjects, but a slight change in mRNA level of SFKs members did not impact on protein levels and protein activity. LPS-induced Erk1/2 phosphorylation was significantly increased in the absence of SFKs activity in active BD patients. However, inhibition of SFKs activity had no effect on LPS-induced phosphorylation of p38 and JNK in both controls and active BD patients.<br />Conclusion: SFKs downregulate LPS-induced Erk1/2 phosphorylation in PBMCs of active BD patients.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Subjects :
- Adult
Case-Control Studies
Cells, Cultured
Female
Gene Expression Regulation, Enzymologic
Humans
Leukocytes, Mononuclear pathology
MAP Kinase Signaling System drug effects
Male
RNA, Messenger genetics
RNA, Messenger metabolism
src-Family Kinases metabolism
Behcet Syndrome blood
Behcet Syndrome genetics
Behcet Syndrome metabolism
Behcet Syndrome pathology
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear metabolism
Lipopolysaccharides
src-Family Kinases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2212-3873
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Endocrine, metabolic & immune disorders drug targets
- Publication Type :
- Academic Journal
- Accession number :
- 30659554
- Full Text :
- https://doi.org/10.2174/1871530319666190119101756