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Regulation of ROS-NF-κB axis by tuna backbone derived peptide ameliorates inflammation in necrotizing enterocolitis.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2019 Aug; Vol. 234 (8), pp. 14330-14338. Date of Electronic Publication: 2019 Jan 17. - Publication Year :
- 2019
-
Abstract
- Necrotizing enterocolitis (NEC) is the most common life-threatening gastrointestinal disease encountered in the premature infant. It has been shown that the intercellular reactive oxygen species (ROS) generation activated by lipopolysaccharide involved in the nuclear factor kappa B (NF-κB) activation and pathogenesis of NEC. Here, we report that an antioxidant peptide from tuna backbone protein (APTBP) reduces the inflammatory cytokines transcription and release. APTBP directly scavenges the free radical through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO) assay. In addition, APTBP reduces the intracellular ROS level, exhibiting an antioxidant activity within cells. Remarkably, gavage with APTBP attenuates the phenotype of NEC in the mice model. Mechanically, the NF-κB activation, together with the expression of inflammatory cytokines are decreased significantly when intracellular ROS are eliminated by APTBP. Therefore, our findings demonstrated that an antioxidant peptide, APTBP, ameliorates inflammation in NEC through attenuating ROS-NF-κB axis.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Animals, Newborn
Biphenyl Compounds pharmacology
Cyclic N-Oxides pharmacology
Disease Models, Animal
Enterocolitis, Necrotizing genetics
Enterocolitis, Necrotizing pathology
Humans
Imidazoles pharmacology
Inflammation genetics
Inflammation pathology
Intestinal Mucosa drug effects
Intestinal Mucosa metabolism
Lipopolysaccharides metabolism
Mice
NF-kappa B genetics
Peptides genetics
Picrates pharmacology
Rats
Reactive Oxygen Species metabolism
Tuna genetics
Cytokines genetics
Enterocolitis, Necrotizing drug therapy
Inflammation drug therapy
Peptides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 234
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 30656693
- Full Text :
- https://doi.org/10.1002/jcp.28133