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Molecular characterization and antioxidant assay of pigment producing bacteria, Sphingomonas paucimobilis and Microbacterium arborescens isolated from fresh water sediments.
- Source :
-
Natural product research [Nat Prod Res] 2020 Apr; Vol. 34 (8), pp. 1192-1196. Date of Electronic Publication: 2019 Jan 14. - Publication Year :
- 2020
-
Abstract
- This study focuses on isolation of pigment producing bacteria from fresh water sediment. The isolated bacteria were grown in nutrient broth and the maximum absorbance of 2.512 was obtained for the extracted pigment at 500 nm. The effective strains were optimized, pH 11 and temperature 30 °C was found to be more favorable for its maximum growth. The isolates were identified based on their molecular characterestics as Microbacterium arborescens and Sphingomonas paucimobilis, molecular size of the amplified 16S rRNA gene sequence was found to be approximately 1270 and 765 bp respectively. The antioxidant property of the pigment was analyzed using DPPH and ABTS assay. The IC <subscript>50</subscript> value of Microbacterium arborescens was higher in all the three assays in comparison with Sphingomonas paucimobilis . The extracted pigment was characterized for the presence of compounds using GC-MS and FTIR analysis to determine the functional groups. As the pigment obtained from M. arborescens had shown better antioxidant activity it may be used as colorant in food industrial applications.
- Subjects :
- Actinomycetales
Antioxidants chemistry
Bacteria cytology
Bacteria genetics
Bacteria isolation & purification
Fresh Water chemistry
Gas Chromatography-Mass Spectrometry
Microbacterium
Phylogeny
Pigments, Biological biosynthesis
RNA, Ribosomal, 16S genetics
Actinobacteria chemistry
Antioxidants isolation & purification
Fresh Water microbiology
Geologic Sediments chemistry
Sphingomonas chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1478-6427
- Volume :
- 34
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Natural product research
- Publication Type :
- Academic Journal
- Accession number :
- 30638407
- Full Text :
- https://doi.org/10.1080/14786419.2018.1553171