Phytotoxic and nematicide evaluation of Croton ehrenbergii (Euphorbiaceae).

Background: Within its natural habitat, Croton ehrenbergii exhibits an innate defense mechanism that is not seen in other plants; it grows unharmed amidst predators and nature, while other species perish. In light of its capacity for defense, C. ehrenbergii was evaluated to better understand the scope of its phytotoxic and nematicide properties. To assess this, fractions obtained by bipartition of a hydro-alcoholic extract, and l-quebrachitol, the main constituent of C. ehrenbergii, were evaluated on Lactuca sativa (dicotyledon) and Lolium perenne (monocotyledon) seeds. Additionally, bipartition fractions and l-quebrachitol were evaluated on a population of Meloidogyne incognita for their nematicide activity.
Results: From this phytochemical research, l-quebrachitol (1), phenylalanine (2), trans-4-hydroxy-N-methylproline (3) and the flavonoids: kaempferol (4), tiliroside (5), nicotiflorine (6) and rutin (7) were identified by spectroscopic analysis. Both methanol and hexane fractions from aerial parts of the plant inhibited the germination and elongation of roots and stems in L. sativa, but not in Lolium perenne, showing that these fractions mostly inhibit the dicotyledon species used in this research. l-Quebrachitol showed slightly higher seed germination inhibition for Lolium perenne in comparison with L. sativa. Three of the fractions evaluated showed nematicide activity against Meloidogyne incognita larvae (J2) at the 48 h benchmark, compared with carbofuran. l-Quebrachitol shows higher mortality after 48 h exposure at a lower concentration than carbofuran.
Conclusion: A variety of compounds were isolated from this research, some were common within the Croton genus (4-7), whereas others were not (1-3). This is the first phytochemical, phytotoxic and nematicide report on C. ehrenbergii. Methanol and hexane fractions from the aerial parts of C. ehrenbergii and l-quebrachitol could be used as alternative natural herbicides, predominantly against dicotyledon weed seeds. All fractions had a good mortality percentage against J2 Meloidogyne incognita larvae. © 2019 Society of Chemical Industry.
(© 2019 Society of Chemical Industry.)