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Molecular damage and responses of oral keratinocyte to hydrogen peroxide.
- Source :
-
BMC oral health [BMC Oral Health] 2019 Jan 11; Vol. 19 (1), pp. 10. Date of Electronic Publication: 2019 Jan 11. - Publication Year :
- 2019
-
Abstract
- Background: Hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> )-based tooth bleaching reagents have recently increased in popularity and controversy. H <subscript>2</subscript> O <subscript>2</subscript> gel (3%) is used in a Nightguard for vital bleaching; transient tooth sensitivity and oral mucosa irritation have been reported. Genotoxicity and carcinogenicity have also been significant concerns.<br />Methods: We used primary cultured normal human oral keratinocytes (NHOKs) as an in vitro model to investigate the pathological effects to mitochondria functions on human oral keratinocytes exposed to different doses of H <subscript>2</subscript> O <subscript>2</subscript> for different durations.<br />Results: An MTT assay showed compromised cell viability at a dose over 5 mM. The treatments induced nuclear DNA damage, measured using a single-cell gel electrophoresis assay. A real-time quantitative polymerase chain reaction showed H <subscript>2</subscript> O <subscript>2</subscript> induced significant increase in mitochondrial 4977-bp deletion. Mitochondrial membrane potential and apoptosis assays suggested that oxidative damage defense mechanisms were activated after prolonged exposure to H <subscript>2</subscript> O <subscript>2</subscript> . Reduced intracellular glutathione was an effective defense against oxidative damage from 5 mM of H <subscript>2</subscript> O <subscript>2</subscript> .<br />Conclusion: Our study suggests the importance for keratinocyte damage of the dose and the duration of the exposure to H <subscript>2</subscript> O <subscript>2</subscript> in at-home-bleaching. A treatment dose ≥100 mM directly causes severe cytotoxicity with as little as 15 min of exposure.
Details
- Language :
- English
- ISSN :
- 1472-6831
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC oral health
- Publication Type :
- Academic Journal
- Accession number :
- 30634966
- Full Text :
- https://doi.org/10.1186/s12903-018-0694-0