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Expression and characterization of an esterase belonging to a new family via isolation from a metagenomic library of paper mill sludge.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2019 Apr 01; Vol. 126, pp. 1192-1200. Date of Electronic Publication: 2019 Jan 06. - Publication Year :
- 2019
-
Abstract
- A new bacterial lipolytic enzyme Est903 was obtained from paper mill sludge via metagenomic approach. Est903 displayed moderate similarities to two lipolytic enzymes from Rhodopirellula islandica and contained a distinctive pentapeptide motif (GFSAG) that differed from those of all known fourteen families of bacterial lipolytic enzymes. Est903 was regarded as from a new bacterial lipolytic enzyme family through multiple sequence alignment and phylogenetic analysis. The recombinant Est903 showed the highest activity for ρ-nitrophenol butyrate. The pH optimum and temperature optimum of the recombinant enzyme was 9.0 and 51 °C, respectively. Also, this enzyme displayed moderate thermostability, high activity under alkaline conditions, and good tolerance against several organic solvents. In addition, the compatibility test and washing performance analysis revealed that Est903 had good compatibility with commercial laundry detergent and high cleaning ability of oil stains. These good properties make Est903 a potential candidate in organic synthesis or detergent industry.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Amino Acid Sequence
Detergents pharmacology
Esterases chemistry
Esterases genetics
Indicators and Reagents
Ions
Metals pharmacology
Phylogeny
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Solutions
Solvents chemistry
Textiles
Esterases isolation & purification
Esterases metabolism
Gene Library
Industrial Waste
Metagenomics
Paper
Sewage
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 126
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 30625356
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.01.025