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Preparation of Zoledronate liposome and its impact on apoptosis of Kupffer cells in rat liver.
- Source :
-
Acta cirurgica brasileira [Acta Cir Bras] 2018 Dec; Vol. 33 (12), pp. 1052-1060. - Publication Year :
- 2018
-
Abstract
- Purpose: To establish a method for the preparation of zoledronate liposome and to observe its effect on inducing the apoptosis of rat liver Kupffer cells.<br />Methods: Zoledronate was encapsulated in liposomes, and then the entrapment rate was detected on a spectrophotometer. The prepared Zoledronate liposome (0.01 mg/mL) was injected into the tail vein of SD rats. Three days later, the number of Kupffer cells (CD68 positive) in rat liver tissue was detected by immunohistochemistry. Flow cytometry was used to detect the apoptosis rate of the isolated liver Kupffer cell cultured in vitro.<br />Results: The entrapment rate of Zoledronate was 43.4±7.8%. Immunohistochemistry revealed that the number of Kupffer cells was 19.3±2.1 in PBS group and 5.5±1.7 in Zoledronate liposome group, with a significant difference (P<0.05). The apoptosis rate of Kupffer cells was 4.1±0.8% in PBS group, while it was 9±2.2% and 23.3±5.9% in Zoledronate liposomes groups with different concentrations of Zoledronate liposome (P<0.05).<br />Conclusions: Zoledronate liposomes can effectively induce the apoptosis of Kupffer cells in vivo and in vitro, and the apoptosis rate is related to the concentration of Zoledronate liposome. To establish a rat liver Kupffer cell apoptosis model can provide a new means for further study on Kupffer cell function.
- Subjects :
- Animals
Cell Count
Drug Compounding methods
Flow Cytometry
Immunohistochemistry
Liposomes chemical synthesis
Male
Random Allocation
Rats, Sprague-Dawley
Reproducibility of Results
Treatment Outcome
Zoledronic Acid administration & dosage
Zoledronic Acid chemical synthesis
Apoptosis drug effects
Kupffer Cells drug effects
Liver cytology
Zoledronic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1678-2674
- Volume :
- 33
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Acta cirurgica brasileira
- Publication Type :
- Academic Journal
- Accession number :
- 30624510
- Full Text :
- https://doi.org/10.1590/s0102-865020180120000002