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Lactobacillus bulgaricus or Lactobacillus rhamnosus Suppresses NF-κB Signaling Pathway and Protects against AFB₁-Induced Hepatitis: A Novel Potential Preventive Strategy for Aflatoxicosis?
- Source :
-
Toxins [Toxins (Basel)] 2019 Jan 04; Vol. 11 (1). Date of Electronic Publication: 2019 Jan 04. - Publication Year :
- 2019
-
Abstract
- Aflatoxin B₁ (AFB₁), a mycotoxin found in food and feed, is immunotoxic to animals and poses significant threat to the food industry and animal production. The primary target of AFB₁ is the liver. To overcome aflatoxin toxicity, probiotic-mediated detoxification has been proposed. In the present study, to investigate the protective effects and molecular mechanisms of Lactobacillus bulgaricus or Lactobacillus rhamnosus against liver inflammatory responses to AFB₁, mice were administered with AFB₁ (300 μg/kg) and/or Lactobacillus intragastrically for 8 weeks. AML12 cells were cultured and treated with AFB₁, BAY 11-7082 (an NF-κB inhibitor), and different concentrations of L. bulgaricus or L. rhamnosus. The body weight, liver index, histopathological changes, biochemical indices, cytokines, cytotoxicity, and activation of the NF-κB signaling pathway were measured. AFB₁ exposure caused changes in liver histopathology and biochemical functions, altered inflammatory response, and activated the NF-κB pathway. Supplementation of L. bulgaricus or L. rhamnosus significantly prevented AFB₁-induced liver injury and alleviated histopathological changes and inflammatory response by decreasing NF-κB p65 expression. The results of in vitro experiments revealed that L. rhamnosus evidently protected against AFB₁-induced inflammatory response and decreased NF-κB p65 expression when compared with L. bulgaricus . These findings indicated that AFB₁ exposure can cause inflammatory response by inducing hepatic injury, and supplementation of L. bulgaricus or L. rhamnosus can produce significant protective effect against AFB₁-induced liver damage and inflammatory response by regulating the activation of the NF-κB signaling pathway.
- Subjects :
- Animals
Cell Line
Chemical and Drug Induced Liver Injury metabolism
Chemical and Drug Induced Liver Injury pathology
Hepatitis metabolism
Hepatitis pathology
Liver metabolism
Liver pathology
Male
Mice
Mycotoxicosis metabolism
Mycotoxicosis pathology
NF-kappa B metabolism
Signal Transduction
Aflatoxin B1
Chemical and Drug Induced Liver Injury prevention & control
Hepatitis prevention & control
Lactobacillus
Mycotoxicosis prevention & control
Probiotics therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 30621122
- Full Text :
- https://doi.org/10.3390/toxins11010017