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Determination of progesterone in saliva using an electrochemical immunosensor and a COTS-based portable potentiostat.
- Source :
-
Analytica chimica acta [Anal Chim Acta] 2019 Feb 21; Vol. 1049, pp. 65-73. Date of Electronic Publication: 2018 Oct 11. - Publication Year :
- 2019
-
Abstract
- This paper describes the reliable determination of progesterone (P4) in undiluted saliva making use of a disposable amperometric immunosensors implemented on low-cost and portable device/potentiostat constructed with commercial-off-the-shelf (COTS) components. The immunosensor allows the fast (45 min), selective and sensitive determination (5 pg mL <superscript>-1</superscript> LOD) of P4 using amperometry in stirred solutions. The immunosensor was coupled to the COTS-based potentiostat and amperometry was made into drops of quiescent solutions. No significant differences were apparent between the analytical performance achieved with the immunosensor for P4 using both a conventional and the COST-based potentiostats. The practical applicability of the immunosensor coupled with the COTS-based potentiostat was demonstrated by determining the endogenous P4 content in different undiluted saliva samples with highly variable endogenous contents of the target hormone. The obtained results were in good agreement with those provided by the conventional ELISA methodology and with the contents reported in the literature for samples with similar characteristics. This validated the combined device for the reliable and minimally invasive determination of the target hormone involving a very simple protocol and taking only 45 min.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Antibodies immunology
Electrochemical Techniques methods
Female
Humans
Immunoassay methods
Limit of Detection
Magnetic Phenomena
Male
Menstrual Cycle metabolism
Progesterone immunology
Progesterone metabolism
Electrochemical Techniques instrumentation
Immunoassay instrumentation
Progesterone analysis
Saliva chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4324
- Volume :
- 1049
- Database :
- MEDLINE
- Journal :
- Analytica chimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 30612658
- Full Text :
- https://doi.org/10.1016/j.aca.2018.10.019