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SubCellBarCode: Proteome-wide Mapping of Protein Localization and Relocalization.
- Source :
-
Molecular cell [Mol Cell] 2019 Jan 03; Vol. 73 (1), pp. 166-182.e7. - Publication Year :
- 2019
-
Abstract
- Subcellular localization is a main determinant of protein function; however, a global view of cellular proteome organization remains relatively unexplored. We have developed a robust mass spectrometry-based analysis pipeline to generate a proteome-wide view of subcellular localization for proteins mapping to 12,418 individual genes across five cell lines. Based on more than 83,000 unique classifications and correlation profiling, we investigate the effect of alternative splicing and protein domains on localization, complex member co-localization, cell-type-specific localization, as well as protein relocalization after growth factor inhibition. Our analysis provides information about the cellular architecture and complexity of the spatial organization of the proteome; we show that the majority of proteins have a single main subcellular location, that alternative splicing rarely affects subcellular location, and that cell types are best distinguished by expression of proteins exposed to the surrounding environment. The resource is freely accessible via www.subcellbarcode.org.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Biomarkers metabolism
Cell Fractionation
Computational Biology
ErbB Receptors antagonists & inhibitors
ErbB Receptors metabolism
Gefitinib pharmacology
Humans
Isoelectric Focusing
MCF-7 Cells
Protein Kinase Inhibitors pharmacology
Protein Transport
Proteins antagonists & inhibitors
Proteins classification
Proteins genetics
Reproducibility of Results
Subcellular Fractions classification
Subcellular Fractions drug effects
Chromatography, Liquid
Mass Spectrometry
Proteins metabolism
Proteome
Proteomics methods
Subcellular Fractions metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 73
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 30609389
- Full Text :
- https://doi.org/10.1016/j.molcel.2018.11.035