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A label-free and highly sensitive DNA biosensor based on the core-shell structured CeO 2 -NR@Ppy nanocomposite for Salmonella detection.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Mar; Vol. 96, pp. 790-797. Date of Electronic Publication: 2018 Nov 28. - Publication Year :
- 2019
-
Abstract
- A core-shell cerium oxide nanorod@polypyrrole (CeO <subscript>2</subscript> -NR@Ppy) nanocomposite-based electrochemical DNA biosensor was studied for Salmonella detection. The core-shell CeO <subscript>2</subscript> -NR@Ppy nanocomposite was prepared by in situ chemical oxidative polymerization of pyrrole monomer on CeO <subscript>2</subscript> -NRs, which provided a suitable platform for electrochemical DNA biosensor fabrication. The immobilization of ss-DNA sequences onto nanocomposite-coated microelectrode was performed via covalent attachment method. DNA biosensor electrochemical responses were studied by cyclic voltammetry and electrochemical impedance spectroscopy with [Fe (CN) <subscript>6</subscript> ] <superscript>3-/4-</superscript> as redox probe. Under optimal conditions, DNA biosensor response showed good linearity in the range of 0.01-0.4 nM with sensitivity of 593.7 Ω·nM <superscript>-1</superscript> ·cm <superscript>-2</superscript> . The low limit of detection and limit of quantification for the DNA biosensor were 0.084 and 0.28 nM, respectively. The proposed DNA biosensor also showed good results when used in detecting actual Salmonella samples.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 96
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 30606592
- Full Text :
- https://doi.org/10.1016/j.msec.2018.11.059