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Extracorporeal shock wave-assisted adipose-derived fresh stromal vascular fraction restores the blood flow of critical limb ischemia in rat.
- Source :
-
Vascular pharmacology [Vascul Pharmacol] 2019 Feb; Vol. 113, pp. 57-69. Date of Electronic Publication: 2018 Dec 28. - Publication Year :
- 2019
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Abstract
- We tested the hypothesis that extracorporeal-shock-wave (ECSW)-assisted adipose-derived stromal vascular fraction (SVF) therapy was better than either one for restoring the blood flow in critical limb ischemia (CLI). Adult male-SD rats were categorized into group 1 (sham-operated-control), group 2 (CLI), group 3 [CLI + ECSW (280 impulses/0.10 mJ/mm <superscript>2</superscript> ) applied to left inguinal area at 3 h after CLI], group 4 [CLI + SVF (1.2 × 10 <superscript>6</superscript> ) implanted into CLI area at 3 h after CLI], group 5 (CLI + ECSW-SVF). In vitro studies showed that ECSW significantly enhanced angiogenesis in human umbilical-vein endothelial cells and carotid-artery ring, and SVF significantly suppressed inflammation (TNF-α/NF-Κb/IL-1ß/MMP-9) in smooth-muscle cells treated by LPS (all p < .001). By day 14 after CLI, the ratio of ischemic/normal blood flow (INBF) was highest in group 1, lowest in group 2, significantly higher in group 5 than in groups 3 and 4, but no difference was shown between the latter two groups (all p < .001). The fibrotic area in CLI region exhibited an opposite pattern of INBF ratio (all p < .0001). Protein (CD31/vWF/eNOS) and cellular (CD31/vWF) expressions and number of small vessels in CLI area exhibited an identical pattern, whilst protein expressions of apoptotic (caspase3/PARP/mitochondrial-Bax) fibrotic/DNA-damaged (Samd3/TFG-ß/γ-H2AX) biomarkers exhibited an opposite pattern to INBF among five groups (all p < .0001). The numbers of angiogenetic cells in CLI region (SDF-1α/VEGF/CXCR4) and endothelial-progenitor cells (C-kit/CD31+//Sca-1/CD31+//CD34/KDR+/VE-cadherin/CD34+) in circulation significantly and progressively increased from groups 2 to 5 (all p < .0001). In conclusion, ECSW-SVF therapy effectively enhanced angiogenesis and restoration of blood flow in CLI area.<br /> (Copyright © 2018. Published by Elsevier Inc.)
- Subjects :
- Angiogenic Proteins metabolism
Animals
Aorta metabolism
Blood Flow Velocity
Carotid Arteries metabolism
Cells, Cultured
Critical Illness
Disease Models, Animal
Endothelial Progenitor Cells metabolism
Hindlimb
Human Umbilical Vein Endothelial Cells metabolism
Humans
Inflammation Mediators metabolism
Ischemia physiopathology
Male
Rats, Sprague-Dawley
Regional Blood Flow
Signal Transduction
Stromal Cells metabolism
Tissue Culture Techniques
Adipose Tissue blood supply
Extracorporeal Shockwave Therapy
Ischemia therapy
Neovascularization, Physiologic
Quadriceps Muscle blood supply
Stromal Cells transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3649
- Volume :
- 113
- Database :
- MEDLINE
- Journal :
- Vascular pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 30597218
- Full Text :
- https://doi.org/10.1016/j.vph.2018.12.003