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Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites.

Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites.

Authors :
Wu H
de Boer R
Krikken AM
Akşit A
Yuan W
van der Klei IJ
Source :
Biochimica et biophysica acta. Molecular cell research [Biochim Biophys Acta Mol Cell Res] 2019 Mar; Vol. 1866 (3), pp. 349-359. Date of Electronic Publication: 2018 Sep 01.
Publication Year :
2019

Abstract

Using electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes and other cell organelles in the yeast Hansenula polymorpha. In exponential glucose-grown cells, which typically contain a single small peroxisome, contacts were only observed with the endoplasmic reticulum and the plasma membrane. Here we focus on a novel peroxisome-vacuole contact site that is formed when glucose-grown cells are shifted to methanol containing media, conditions that induce strong peroxisome development. At these conditions, the small peroxisomes rapidly increase in size, a phenomenon that is paralleled by the formation of distinct intimate contacts with the vacuole. Localization studies showed that the peroxin Pex3 accumulated in patches at the peroxisome-vacuole contact sites. In wild-type cells growing exponentially on medium containing glucose, peroxisome-vacuole contact sites were never observed. However, upon overproduction of Pex3 peroxisomes also associated to vacuoles at these growth conditions. Our observations strongly suggest a role for Pex3 in the formation of a novel peroxisome-vacuole contact site. This contact likely plays a role in membrane growth as it is formed solely at conditions of strong peroxisome expansion.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1879-2596
Volume :
1866
Issue :
3
Database :
MEDLINE
Journal :
Biochimica et biophysica acta. Molecular cell research
Publication Type :
Academic Journal
Accession number :
30595161
Full Text :
https://doi.org/10.1016/j.bbamcr.2018.08.021