Back to Search
Start Over
A new seed-transmissible begomovirus in bitter gourd (Momordica charantia L.).
- Source :
-
Microbial pathogenesis [Microb Pathog] 2019 Mar; Vol. 128, pp. 82-89. Date of Electronic Publication: 2018 Dec 21. - Publication Year :
- 2019
-
Abstract
- A begomovirus isolate collected from bitter gourd plants showing yellowing, puckering and stunting symptoms from Coimbatore district, Tamil Nadu, India was characterized. The full-length genome of the virus isolate was amplified by rolling circle amplification using phi29 DNA polymerase. The virus isolate exhibited 98% identity in the nucleotide sequence of DNA-A component with the Coccinia mosaic Virudhunagar virus (GenBank accession no. KY860899). The DNA-B component was very distinct and shared only 60% identity with the begomovirus, Coccinia mosaic Tamil Nadu virus (GenBank accession no. KM244719). The virus renamed as new species Bitter gourd yellow mosaic virus (BgYMV) was detected in seeds from infected plants and in the grow-out test seedlings by ELISA and virus-specific PCR. The seed infectivity was 79.16% and transmission rate to seedling was 32.05%. The virus titre as indicated by A <subscript>405</subscript> absorption value was high (0.854-0.280) in different seed parts. Results clearly indicated seed transmission of the begomovirus, BgYMV.<br /> (Copyright © 2018. Published by Elsevier Ltd.)
- Subjects :
- Base Sequence
Cloning, Molecular
DNA, Viral
Fruit
Genome, Viral
India
Plant Leaves virology
Seedlings virology
Sequence Analysis, DNA
Species Specificity
Begomovirus classification
Begomovirus genetics
Begomovirus isolation & purification
Disease Transmission, Infectious
Momordica charantia virology
Phylogeny
Plant Diseases virology
Seeds virology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 128
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 30583019
- Full Text :
- https://doi.org/10.1016/j.micpath.2018.12.036