Back to Search
Start Over
Recombinant BCG Vaccines Reduce Pneumovirus-Caused Airway Pathology by Inducing Protective Humoral Immunity.
- Source :
-
Frontiers in immunology [Front Immunol] 2018 Dec 06; Vol. 9, pp. 2875. Date of Electronic Publication: 2018 Dec 06 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- The Human Respiratory Syncytial Virus (hRSV) and the Human Metapneumovirus (hMPV) are two pneumoviruses that are leading agents causing acute lower respiratory tract infections (ALRTIs) affecting young infants, the elderly, and immunocompromised patients worldwide. Since these pathogens were first discovered, many approaches for the licensing of safe and effective vaccines have been explored being unsuccessful to date. We have previously described that immunization with recombinant strains of Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) expressing the hRSV nucleoprotein (rBCG-N) or the hMPV phosphoprotein (rBCG-P) induced immune protection against each respective virus. These vaccines efficiently promoted viral clearance without significant lung damage, mainly through the induction of a T helper 1 cellular immunity. Here we show that upon viral challenge, rBCG-immunized mice developed a protective humoral immunity, characterized by production of antibodies specific for most hRSV and hMPV proteins. Further, isotype switching from IgG1 to IgG2a was observed in mice immunized with rBCG vaccines and correlated with an increased viral clearance, as compared to unimmunized animals. Finally, sera obtained from animals immunized with rBCG vaccines and infected with their respective viruses exhibited virus neutralizing capacity and protected naïve mice from viral replication and pulmonary disease. These results support the notion that the use of rBCG strains could be considered as an effective vaccination approach against other respiratory viruses with similar biology as hRSV and hMPV.
- Subjects :
- Animals
BCG Vaccine administration & dosage
Cell Line, Tumor
Disease Models, Animal
Humans
Metapneumovirus genetics
Metapneumovirus immunology
Mice
Mice, Inbred BALB C
Nucleoproteins administration & dosage
Nucleoproteins genetics
Nucleoproteins immunology
Recombinant Proteins administration & dosage
Recombinant Proteins genetics
Recombinant Proteins immunology
Respiratory Syncytial Virus, Human genetics
Respiratory Syncytial Virus, Human immunology
Respiratory Tract Infections immunology
Respiratory Tract Infections virology
Th1 Cells immunology
Th1 Cells metabolism
Vaccination methods
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic immunology
Viral Proteins administration & dosage
Viral Proteins genetics
Viral Proteins immunology
Virus Replication immunology
BCG Vaccine immunology
Immunity, Humoral
Mycobacterium bovis immunology
Respiratory Tract Infections prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 30581437
- Full Text :
- https://doi.org/10.3389/fimmu.2018.02875