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The Wnt modulator ICG‑001 mediates the inhibition of nasopharyngeal carcinoma cell migration in vitro via the miR‑150/CD44 axis.
- Source :
-
International journal of oncology [Int J Oncol] 2019 Mar; Vol. 54 (3), pp. 1010-1020. Date of Electronic Publication: 2018 Dec 12. - Publication Year :
- 2019
-
Abstract
- The Wnt signaling pathway is known to serve an important role in the control of cell migration. The present study analyzed the mechanisms underlying the in vitro modulation of the migration of nasopharyngeal carcinoma (NPC) cells by the CREB‑binding protein/catenin antagonist and Wnt modulator ICG‑001. The results revealed that ICG‑001‑mediated inhibition of tumor cell migration involved downregulated mRNA and protein expression of the Wnt target gene cluster of differentiation (CD)44. It was also demonstrated that ICG‑001 downregulated the expression of CD44, and this effect was accompanied by restored expression of microRNA (miRNA)‑150 in various NPC cell lines. Using a CD44 3'‑untranslated region luciferase reporter assay, miR‑150 was confirmed to be a novel CD44‑targeting miRNA, which could directly target CD44 and subsequently regulate the migration of NPC cells. The present study provides further insight into the inhibition of tumor cell migration through the modulation of miRNA expression by the Wnt modulator ICG‑001.
- Subjects :
- Animals
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic drug effects
Humans
Hyaluronan Receptors antagonists & inhibitors
Hyaluronan Receptors metabolism
Mice
MicroRNAs metabolism
Nasopharyngeal Carcinoma genetics
Nasopharyngeal Carcinoma pathology
RNA, Messenger metabolism
RNA, Small Interfering
Bridged Bicyclo Compounds, Heterocyclic pharmacology
Cell Movement drug effects
Hyaluronan Receptors genetics
MicroRNAs genetics
Nasopharyngeal Carcinoma metabolism
Pyrimidinones pharmacology
Wnt Signaling Pathway drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 54
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 30569106
- Full Text :
- https://doi.org/10.3892/ijo.2018.4664