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miR‑107‑5p promotes tumor proliferation and invasion by targeting estrogen receptor‑α in endometrial carcinoma.
- Source :
-
Oncology reports [Oncol Rep] 2019 Mar; Vol. 41 (3), pp. 1575-1585. Date of Electronic Publication: 2018 Dec 18. - Publication Year :
- 2019
-
Abstract
- The aberrant expression of miR107‑5p is closely related to the development of several types of human cancers. However, the role of miR‑107‑5p in endometrial carcinoma (EC) has not been fully confirmed. In the present study, we aimed to explore the function of miR‑107‑5p in EC carcinogenesis. EC samples and normal endometrial tissues were obtained by laser capture microdissection. It was determined that the expression of miR‑107‑5p in EC was significantly higher than that in normal endometrium, and higher miR‑107‑5p expression was related to advanced FIGO stages, lymph node metastasis and myometrial invasion in EC patients. Blocking miR‑107‑5p significantly inhibited cell proliferation, migration and invasion of EC cells in vitro and in vivo. The results of bioinformatic algorithms and luciferase reporter assays revealed that estrogen receptor α (ERα) was a direct target of miR‑107‑5p. miR‑107‑5p downregulated the expression of ERα mRNA and protein. In conclusion, our results highlighted that miR‑107‑5p is a novel prognostic factor that targets ERα to promote tumor proliferation and invasion of EC.
- Subjects :
- 3' Untranslated Regions genetics
Animals
Cell Line, Tumor
Cell Proliferation genetics
Computational Biology
Down-Regulation
Endometrial Neoplasms pathology
Endometrium pathology
Estrogen Receptor alpha metabolism
Female
HEK293 Cells
Humans
Lymphatic Metastasis genetics
Lymphatic Metastasis pathology
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs genetics
Middle Aged
Myometrium pathology
Neoplasm Invasiveness genetics
Prognosis
RNA, Messenger metabolism
Xenograft Model Antitumor Assays
Carcinogenesis genetics
Endometrial Neoplasms genetics
Estrogen Receptor alpha genetics
Gene Expression Regulation, Neoplastic
MicroRNAs metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 41
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 30569100
- Full Text :
- https://doi.org/10.3892/or.2018.6936