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Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method.
Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method.
- Source :
-
American journal of veterinary research [Am J Vet Res] 1988 Oct; Vol. 49 (10), pp. 1719-21. - Publication Year :
- 1988
-
Abstract
- An indirect immunoperoxidase staining technique was evaluated for detection of Mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. Lungs from swine with induced (n = 4) or naturally occurring M hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against M hyopneumoniae as the primary antibody. Organisms stained by the indirect immunoperoxidase method were identified in tissue sections as pleomorphic brown-staining structures corresponding to those observed with immunofluorescence. Mycoplasma hyosynoviae, M hyorhinis, and Acholeplasma laidlawii did not stain with the indirect immunoperoxidase method, using antibody raised against M hyopneumoniae.
- Subjects :
- Animals
Female
Fluorescent Antibody Technique
Immunoenzyme Techniques
Lung pathology
Male
Mycoplasma Infections diagnosis
Mycoplasma Infections microbiology
Pneumonia diagnosis
Pneumonia microbiology
Predictive Value of Tests
Swine
Swine Diseases diagnosis
Lung microbiology
Mycoplasma isolation & purification
Mycoplasma Infections veterinary
Pneumonia veterinary
Swine Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9645
- Volume :
- 49
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 3056138