Molecular characterization of virulence and antibiotic resistance genes among Salmonella serovars isolated from broilers in Egypt.

This study was carried out to investigate the resistance to some antimicrobial groups among Salmonellae isolated from broilers in Egypt. The prevalence of some virulence and resistance genes among the recovered multidrug resistant (MDR) isolates was also scrutinized. A total of 55 (15·6%) Salmonella isolates were recovered from 353 different samples (liver, yolk sac, gall bladder and caecum), gathered from apparently healthy and diseased broilers suffered from diarrhoea, dehydration and respiratory distress. Thirty Salmonellae strains were serotyped into Salmonella Enteritidis, Salmonella Infantis, Salmonella Kentucky, Salmonella Maloma, Salmonella Bardo, Salmonella Gdansk, Salmonella Typhimurium and Salmonella Blegdame. The resistance pattern of all Salmonella isolates was constructed and 15 MDR Salmonella isolates were subsequently examined for the presence of virulence (invA, ompA and stn) and resistance (qnrS, qnrA, bla _TEM and bla _CTX ) genes. Of note, invA, ompA and stn virulence genes and bla _TEM and qnrS resistance genes were found in all examined isolates. On the other hand, the qnrA gene detection frequency was 20%, whereas bla _CTX was not detected at all. Our findings emphasize the wide spread of antimicrobial resistance genes in Salmonella isolates and the importance of effective control measures for the disease. SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights the possible emergence of widespread resistance to some antimicrobials among Salmonellae isolated from broilers in Egypt. The results also reveal the prevalence of some virulence (invA, ompA and stn) and resistance (qnrS, qnrA, bla _CTX and bla _TEM ) genes among the recovered multidrug resistant isolates. Clearly, our data emphasize that antimicrobial resistance genes are widely spread in Salmonella isolates which is crucial for developing novel methods for controlling such disease problem of zoonotic concern.
(© 2018 The Society for Applied Microbiology.)