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l-Theanine and NEP1-40 promote nerve regeneration and functional recovery after brachial plexus root avulsion.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Jan 22; Vol. 508 (4), pp. 1126-1132. Date of Electronic Publication: 2018 Dec 12. - Publication Year :
- 2019
-
Abstract
- Brachial plexus root avulsion causes severe sequelae Treatments and prognosis face many problems, including inflammatory reaction, oxidative damage, and myelin related inhibitory effect. l-Theanine has anti-inflammatory, anti-oxidative, and neuroprotective effects. NEP1-40 competitively inhibits Nogo-66 receptor (NgR1) promotes axonal regeneration. Forty-eight Sprague-Dawley rats were randomly assigned into four groups to establish an animal model of brachial plexus root avulsion. Inflammation and oxidative damage were evaluated by spectrophotometry and motor function of the upper limbs was assessed via Terzis grooming test after modeling. Immunofluorescence and hematoxylin and eosin staining were utilized to determine the content of reactive oxygen species, activation of microglial cells, neuroprotection, and nerve regeneration. Compared with the control group, the L-Theanine + NEP1-40 group had significantly decreased myeloperoxidase, malondialdehyde, interleukin-6, reactive oxygen species, and microglial cells, significantly increased score on the Terzis grooming test, increased motor neuron content, and thickened muscle fibers, increased area, and appearance of large and clear motor endplate structures. The results of this study suggest that l-Theanine combined with NEP1-40significantly promoted nerve regeneration after brachial plexus root avulsion, and may be a potential treatment for promoting nerve regeneration. Possible mechanisms underlying these results are alleviation of oxidative damage and inflammatory responses in the injured area and antagonism of myelin inhibition.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Anterior Horn Cells drug effects
Anterior Horn Cells metabolism
Anterior Horn Cells pathology
Brachial Plexus drug effects
Brachial Plexus pathology
Cell Survival drug effects
Drug Therapy, Combination
Female
Glutamates pharmacology
Interleukin-6 metabolism
Malondialdehyde metabolism
Microglia drug effects
Microglia metabolism
Motor Endplate drug effects
Motor Endplate physiopathology
Motor Neurons drug effects
Motor Neurons metabolism
Motor Neurons pathology
Muscle Fibers, Skeletal drug effects
Muscle Fibers, Skeletal metabolism
Peptide Fragments pharmacology
Peroxidase metabolism
Radiculopathy pathology
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Spinal Cord drug effects
Spinal Cord metabolism
Spinal Cord pathology
Spinal Cord physiopathology
Brachial Plexus injuries
Brachial Plexus physiopathology
Glutamates therapeutic use
Nerve Regeneration drug effects
Peptide Fragments therapeutic use
Radiculopathy drug therapy
Radiculopathy physiopathology
Recovery of Function drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 508
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 30553451
- Full Text :
- https://doi.org/10.1016/j.bbrc.2018.11.124