Bacterial Resistance and Prostate Cancer Susceptibility Toward Metal-Ion-doped DNA Complexes.

DNA nanotechnology has laid a platform to construct a variety of custom-shaped nanoscale objects for functionalization of specific target materials to achieve programmability and molecular recognition. Herein, we prepared DNA nanostructures [namely, synthetic DNA rings (RDNA) and DNA duplexes extracted from salmon (SDNA)] containing metal ions (M ²⁺ ) such as Cu ²⁺ , Ni ²⁺ , and Zn ²⁺ as payloads for delivery to exterminate highly pathologic hospital bacterial strains (e.g., Escherichia coli and Bacillus subtilis) and prostate cancer cells (i.e., PC3, LNCaP, TRAMP-C1, 22Rv1, and DU145). Morphologies of these M ²⁺ -doped RDNA were visualized using atomic force microscopy. Interactions between M ²⁺ and DNA were studied using UV-vis and Fourier transform infrared spectroscopy. Quantitative composition and chemical changes in DNA without or with M ²⁺ were obtained using X-ray photoelectron spectroscopy. In addition, M ²⁺ -doped DNA complexes were subjected to antibacterial activity studies. They showed no bacteriostatic or bactericidal effects on bacterial strains used. Finally, in vitro cellular toxicity study was conducted to evaluate the effect of pristine DNA and M ²⁺ -doped DNA complexes on prostate cancer cells. Cytotoxicities conferred by M ²⁺ -doped DNA complexes for most cell lines were significantly higher than those of M ²⁺ without DNA. Cellular uptake of these complexes was confirmed by fluorescence microscopy using PhenGreen FL indicator. On the basis of our observations, DNA nanostructures can be used as safe and efficient nanocarriers for delivery of therapeutics. They have enhanced therapeutic window than bare metals.