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MicroRNA-1204 promotes cell proliferation by regulating PITX1 in non-small-cell lung cancer.
- Source :
-
Cell biology international [Cell Biol Int] 2019 Mar; Vol. 43 (3), pp. 253-264. Date of Electronic Publication: 2019 Jan 28. - Publication Year :
- 2019
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Abstract
- MicroRNA-1204 (miR-1204), a member of the PVT1 region, may improve B cell differentiation and metastasis in breast cancer. However, the role of miR-1204 in non-small-cell lung cancer (NSCLC) and its mechanism remain unclear. The GEO public database was first employed to find differentially expressed genes. The expression level of miR-1204 in patient tissues and NSCLC cell lines was determined using qRT-PCR. Cell proliferation assays were performed to investigate the impact of miR-1204 on cell growth. Bioinformatics analysis and dual-luciferase reporter assays were conducted to find potential target genes. Finally, we performed in vivo experiments to identify the effect of miR-1204 on tumor formation in nude mice. It was first found that miR-1204 was overexpressed in NSCLC tissues and cells. miR-1204 increased the proliferation of NSCLC cells and reduced cell cycle arrest in vitro. PITX1 (paired like homeodomain 1) was found as a potential target gene. In addition, PITX1 was also found to be low in expression in NSCLC tissues and cells. To show that PITX1 reversed the function of miR-1204 in promoting proliferation, confirmatory experiments were performed. Moreover, high miR-1204 and low PITX1 expression was highly correlated with tumor size, lymph node metastasis, and the TNM stage in patients diagnosed with NSCLC. Our results suggested that upregulated miR-1204 in NSCLC is associated with NSCLC progression and promotes NSCLC cell proliferation by downregulating PITX1. miR-1204 may act as a poor prognostic factor and a potential therapeutic target for NSCLC.<br /> (© 2018 International Federation for Cell Biology.)
- Subjects :
- 3' Untranslated Regions genetics
Animals
Carcinogenesis genetics
Carcinogenesis pathology
Cell Line, Tumor
Cell Proliferation genetics
Female
Humans
Male
Mice, Nude
MicroRNAs genetics
Middle Aged
Paired Box Transcription Factors metabolism
Up-Regulation genetics
Xenograft Model Antitumor Assays
Carcinoma, Non-Small-Cell Lung genetics
Carcinoma, Non-Small-Cell Lung pathology
Gene Expression Regulation, Neoplastic
Lung Neoplasms genetics
Lung Neoplasms pathology
MicroRNAs metabolism
Paired Box Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8355
- Volume :
- 43
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell biology international
- Publication Type :
- Academic Journal
- Accession number :
- 30549141
- Full Text :
- https://doi.org/10.1002/cbin.11083