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Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1901, pp. 79-87. - Publication Year :
- 2019
-
Abstract
- Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1901
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 30539569
- Full Text :
- https://doi.org/10.1007/978-1-4939-8949-2_6