Structure elucidation and gene cluster annotation of the O-antigen of Vibrio cholerae O100 containing two rarely occurred amino sugar derivatives.

Authors :: Perepelov AV
Guo X
Filatov AV
Shashkov AS
Senchenkova SN
Li B
Source :: Carbohydrate research [Carbohydr Res] 2019 Jan 15; Vol. 472, pp. 98-102. Date of Electronic Publication: 2018 Nov 05.
Publication Year :: 2019
Abstract: O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Vibrio cholerae O100 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: →3)-β-d-QuipNAc4N(dHh)-(1 → 3)-α-d-Fucp4N(RHb)-(1 → 3)-α-l-FucpNAc-(1→ where Hb and dHh indicate 3-hydroxybutanoyl and 3,5-dihydroxyhexanoyl, respectively. The O-antigen gene cluster of V. cholerae O100 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Subjects :: Amino Sugars chemistry
Carbohydrate Sequence
Models, Molecular
Molecular Sequence Annotation
Multigene Family
Nuclear Magnetic Resonance, Biomolecular
Vibrio cholerae chemistry
Vibrio cholerae genetics
O Antigens chemistry
O Antigens genetics
Sequence Analysis, DNA methods
Vibrio cholerae metabolism

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