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Quantitative Detection of Fipronil and Fipronil-Sulfone in Sera of Black-Tailed Prairie Dogs and Rats after Oral Exposure to Fipronil by Camel Single-Domain Antibody-Based Immunoassays.
- Source :
-
Analytical chemistry [Anal Chem] 2019 Jan 15; Vol. 91 (2), pp. 1532-1540. Date of Electronic Publication: 2018 Dec 21. - Publication Year :
- 2019
-
Abstract
- The insecticide fipronil can be metabolized to its sulfone in mammalian species. Two camel single-domain antibodies (VHHs) F1 and F6, selective to fipronil and fipronil-sulfone, respectively, were generated and used to develop enzyme linked immunosorbent assays (ELISAs) for the detection of the two compounds in the sera of black-tailed prairie dogs and rats. The limits of detection of fipronil and fipronil-sulfone in the rodent sera by the corresponding ELISAs were 10 and 30 ng mL <superscript>-1</superscript> , and the linear ranges were 30-1000 and 75-2200 ng mL <superscript>-1</superscript> . ELISAs showed a good recovery for fipronil and fipronil-sulfone cospiked in the control sera of the black-tailed prairie dogs (90-109%) and rats (93-106%). The VHH-based ELISAs detected fipronil and fipronil-sulfone in the sera of the rodents that received a repeated oral administration of fipronil. The average concentration of fipronil-sulfone was approximately 3.2-fold higher than fipronil in the prairie dog sera (1.15 vs 0.36 μg mL <superscript>-1</superscript> ) and rat sera (1.77 vs 0.53 μg mL <superscript>-1</superscript> ). ELISAs agreed well with a liquid chromatography-mass spectrometry method for the quantification of both fipronil and fipronil-sulfone in real serum samples. Fipronil-sulfone was identified as the predominant metabolite of fipronil in the black-tailed prairie dog and rat sera.
- Subjects :
- Administration, Oral
Animals
Immunization
Insecticides administration & dosage
Insecticides immunology
Insecticides metabolism
Pyrazoles administration & dosage
Pyrazoles immunology
Pyrazoles metabolism
Rats
Sciuridae
Enzyme-Linked Immunosorbent Assay methods
Insecticides blood
Pyrazoles blood
Single-Domain Antibodies immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 91
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 30521755
- Full Text :
- https://doi.org/10.1021/acs.analchem.8b04653