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Role of FGF signalling in neural crest cell migration during early chick embryo development.

Authors :
Zhang XT
Wang G
Li Y
Chuai M
Lee KKH
Yang X
Source :
Zygote (Cambridge, England) [Zygote] 2018 Dec; Vol. 26 (6), pp. 457-464. Date of Electronic Publication: 2018 Dec 06.
Publication Year :
2018

Abstract

SummaryFibroblast growth factor (FGF) signalling acts as one of modulators that control neural crest cell (NCC) migration, but how this is achieved is still unclear. In this study, we investigated the effects of FGF signalling on NCC migration by blocking this process. Constructs that were capable of inducing Sprouty2 (Spry2) or dominant-negative FGFR1 (Dn-FGFR1) expression were transfected into the cells making up the neural tubes. Our results revealed that blocking FGF signalling at stage HH10 (neurulation stage) could enhance NCC migration at both the cranial and trunk levels in the developing embryos. It was established that FGF-mediated NCC migration was not due to altering the expression of N-cadherin in the neural tube. Instead, we determined that cyclin D1 was overexpressed in the cranial and trunk levels when Sprouty2 was upregulated in the dorsal neural tube. These results imply that the cell cycle was a target of FGF signalling through which it regulates NCC migration at the neurulation stage.

Details

Language :
English
ISSN :
1469-8730
Volume :
26
Issue :
6
Database :
MEDLINE
Journal :
Zygote (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
30520400
Full Text :
https://doi.org/10.1017/S096719941800045X