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Role of FGF signalling in neural crest cell migration during early chick embryo development.
- Source :
-
Zygote (Cambridge, England) [Zygote] 2018 Dec; Vol. 26 (6), pp. 457-464. Date of Electronic Publication: 2018 Dec 06. - Publication Year :
- 2018
-
Abstract
- SummaryFibroblast growth factor (FGF) signalling acts as one of modulators that control neural crest cell (NCC) migration, but how this is achieved is still unclear. In this study, we investigated the effects of FGF signalling on NCC migration by blocking this process. Constructs that were capable of inducing Sprouty2 (Spry2) or dominant-negative FGFR1 (Dn-FGFR1) expression were transfected into the cells making up the neural tubes. Our results revealed that blocking FGF signalling at stage HH10 (neurulation stage) could enhance NCC migration at both the cranial and trunk levels in the developing embryos. It was established that FGF-mediated NCC migration was not due to altering the expression of N-cadherin in the neural tube. Instead, we determined that cyclin D1 was overexpressed in the cranial and trunk levels when Sprouty2 was upregulated in the dorsal neural tube. These results imply that the cell cycle was a target of FGF signalling through which it regulates NCC migration at the neurulation stage.
- Subjects :
- Animals
Cadherins genetics
Cadherins metabolism
Cell Movement
Cyclin D1 genetics
Cyclin D1 metabolism
Gene Expression Regulation, Developmental
Nerve Tissue Proteins genetics
Nerve Tissue Proteins metabolism
Neural Crest metabolism
Neural Tube embryology
Neural Tube metabolism
Receptor, Fibroblast Growth Factor, Type 1 genetics
Receptor, Fibroblast Growth Factor, Type 1 metabolism
Signal Transduction
Chick Embryo cytology
Chick Embryo metabolism
Fibroblast Growth Factors metabolism
Neural Crest cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8730
- Volume :
- 26
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Zygote (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 30520400
- Full Text :
- https://doi.org/10.1017/S096719941800045X