Back to Search
Start Over
ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier.
- Source :
-
Developmental cell [Dev Cell] 2018 Dec 17; Vol. 47 (6), pp. 741-757.e8. Date of Electronic Publication: 2018 Nov 29. - Publication Year :
- 2018
-
Abstract
- Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity to adapt to mechanical requirements of the filtration barrier. We find that N-WASP-Arp2/3 define the development of complex arborized podocyte protrusions in vitro and in vivo. Loss of dendritic actin networks results in a pronounced activation of the actomyosin cytoskeleton and the generation of over-maturated but less efficient adhesion, leading to detachment of podocytes. Our data provide a model to explain podocyte protrusion morphology and their mechanical stability based on a tripartite relationship between actin polymerization, contractility, and adhesion.<br /> (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Actin Cytoskeleton metabolism
Actin-Related Protein 3 metabolism
Actins metabolism
Actomyosin metabolism
Animals
Cell Adhesion
Focal Adhesions metabolism
Glomerular Filtration Barrier metabolism
Humans
Kidney metabolism
Kidney physiology
Mice
Mice, Knockout
Podocytes metabolism
Signal Transduction
Wiskott-Aldrich Syndrome Protein, Neuronal metabolism
Actin-Related Protein 3 physiology
Glomerular Filtration Barrier physiology
Podocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 47
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 30503751
- Full Text :
- https://doi.org/10.1016/j.devcel.2018.11.011