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Ectopic Organogenesis after Allotransplantation of Freshly Removed or Cryopreserved Neonatal Testicle under the Renal Capsule in Rats.
- Source :
-
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2018 Dec; Vol. 166 (2), pp. 268-273. Date of Electronic Publication: 2018 Nov 28. - Publication Year :
- 2018
-
Abstract
- In experiments on white outbred male rats, a freshly removed (20 experiments) or cryopreserved (10 experiments) testicle from newborn rats (1-2 days after birth) was transplanted under the renal capsule after bilateral orchiectomy. In all experiments with transplantation of freshly removed testicle, it was engrafted. In 3 months, histological examination revealed the formation of mature seminiferous tubules, but spermatogenesis was blocked at the stage of spermatogonia; groups of proliferating Leydig cells in the loose connective tissue between the tubules were also seen. In 6 and 12 months, the status of the seminiferous tubules remained unchanged, but structures typical of the epididymis and developing vas deferens were revealed. The number of proliferating Leydig cells increased. The initially low testosterone concentration in the blood of castrated males increased significantly as soon as in 1 month after transplantation and grew up to 3 months, remaining at a level ~50% of normal. Engraftment of cryopreserved neonatal testicular tissue was observed in 60% cases, however, engrafted tissue, similar to the fresh one, retained the ability for organogenesis with the formation of mature seminiferous tubules, epididymis, and groups of proliferating Leydig cells. The dynamics of blood testosterone concentration in rats with cryopreserved and fresh transplantation was similar. Subcapsular transplantation did not adversely affect the kidneys, which was seen from normal histological structure of the kidneys and creatinine and urea concentrations in the blood.
- Subjects :
- Animals
Animals, Newborn
Animals, Outbred Strains
Creatinine blood
Cryoprotective Agents pharmacology
Dimethyl Sulfoxide pharmacology
Epididymis cytology
Epididymis physiology
Kidney
Kidney Function Tests
Leydig Cells cytology
Leydig Cells physiology
Male
Rats
Regenerative Medicine methods
Seminiferous Tubules cytology
Seminiferous Tubules physiology
Testis cytology
Testis growth & development
Testis surgery
Testosterone blood
Transplantation, Heterotopic
Transplantation, Homologous
Urea blood
Cryopreservation methods
Graft Survival
Orchiectomy
Organogenesis
Testis transplantation
Testosterone biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1573-8221
- Volume :
- 166
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bulletin of experimental biology and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 30488204
- Full Text :
- https://doi.org/10.1007/s10517-018-4330-4