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Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells.
- Source :
-
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2018 Dec; Vol. 166 (2), pp. 264-267. Date of Electronic Publication: 2018 Nov 28. - Publication Year :
- 2018
-
Abstract
- We studied the influence of magnetite nanoparticles (FeO•Fe <subscript>2</subscript> O <subscript>3</subscript> ) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.
- Subjects :
- 14-3-3 Proteins metabolism
3-Mercaptopropionic Acid chemistry
Actins genetics
Actins metabolism
Cadmium Compounds pharmacology
Ferric Compounds pharmacology
Humans
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear metabolism
Peptidylprolyl Isomerase genetics
Peptidylprolyl Isomerase metabolism
Primary Cell Culture
Reference Standards
Selenium Compounds pharmacology
Sulfides pharmacology
Ubiquitin C genetics
Ubiquitin C metabolism
Zinc Compounds pharmacology
beta 2-Microglobulin genetics
beta 2-Microglobulin metabolism
14-3-3 Proteins genetics
Gene Expression Regulation drug effects
Leukocytes, Mononuclear drug effects
Magnetite Nanoparticles chemistry
Polymerase Chain Reaction standards
Quantum Dots chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1573-8221
- Volume :
- 166
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bulletin of experimental biology and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 30488202
- Full Text :
- https://doi.org/10.1007/s10517-018-4329-x