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Induction of Apoptosis and Cytotoxicity by Raphasatin in Human Breast Adenocarcinoma MCF-7 Cells.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2018 Nov 27; Vol. 23 (12). Date of Electronic Publication: 2018 Nov 27. - Publication Year :
- 2018
-
Abstract
- Glucoraphasatin (GRH), a glucosinolate present abundantly in the plants of the Brassicaceae family, is hydrolyzed by myrosinase to raphasatin, which is considered responsible for its cancer chemopreventive activity; however, the underlying mechanisms of action have not been investigated, particularly in human cell lines. The aims of this study are to determine the cytotoxicity of raphasatin, and to evaluate its potential to cause apoptosis and modulate cell cycle arrest in human breast adenocarcinoma MCF-7 cells. The cytotoxicity was determined following incubation of the cells with glucoraphasatin or raphasatin (0⁻100 µM), for 24, 48, and 72 h. GRH displayed no cytotoxicity as exemplified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When myrosinase was added to the incubation system to convert GRH to raphasatin, cytotoxicity was evident. Exposure of the cells to raphasatin stimulated apoptosis, as was exemplified by cell shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. Moreover, using Annexin V-FITC assay, raphasatin induced apoptosis, as witnessed by changes in cellular distribution of cells, at different stages of apoptosis; in addition, raphasatin caused the arrest of the MCF-7 cells at the G₂ + M phase. In conclusion, raphasatin demonstrated cancer chemopreventive potential against human breast adenocarcinoma (MCF-7) cells, through induction of apoptosis and cell cycle arrest.
- Subjects :
- Adenocarcinoma drug therapy
Adenocarcinoma pathology
Breast Neoplasms drug therapy
Breast Neoplasms pathology
Cytotoxins chemistry
Female
G2 Phase Cell Cycle Checkpoints drug effects
Humans
Isothiocyanates chemistry
M Phase Cell Cycle Checkpoints drug effects
MCF-7 Cells
Adenocarcinoma metabolism
Apoptosis drug effects
Breast Neoplasms metabolism
Cytotoxins pharmacology
Isothiocyanates pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 23
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 30486382
- Full Text :
- https://doi.org/10.3390/molecules23123092