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Imaging Cortical Dynamics in GCaMP Transgenic Rats with a Head-Mounted Widefield Macroscope.
- Source :
-
Neuron [Neuron] 2018 Dec 05; Vol. 100 (5), pp. 1045-1058.e5. Date of Electronic Publication: 2018 Oct 25. - Publication Year :
- 2018
-
Abstract
- Widefield imaging of calcium dynamics is an emerging method for mapping regional neural activity but is currently limited to restrained animals. Here we describe cScope, a head-mounted widefield macroscope developed to image large-scale cortical dynamics in rats during natural behavior. cScope provides a 7.8 × 4 mm field of view and dual illumination paths for both fluorescence and hemodynamic correction and can be fabricated at low cost using readily attainable components. We also report the development of Thy-1 transgenic rat strains with widespread neuronal expression of the calcium indicator GCaMP6f. We combined these two technologies to image large-scale calcium dynamics in the dorsal neocortex during a visual evidence accumulation task. Quantitative analysis of task-related dynamics revealed multiple regions having neural signals that encode behavioral choice and sensory evidence. Our results provide a new transgenic resource for calcium imaging in rats and extend the domain of head-mounted microscopes to larger-scale cortical dynamics. VIDEO ABSTRACT.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Behavior, Animal
Calcium analysis
Electrophysiology instrumentation
Electrophysiology methods
Female
Luminescent Proteins genetics
Male
Microscopy instrumentation
Optical Imaging instrumentation
Rats, Transgenic
Calcium Signaling
Microscopy methods
Neocortex physiology
Neurons physiology
Optical Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4199
- Volume :
- 100
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 30482694
- Full Text :
- https://doi.org/10.1016/j.neuron.2018.09.050