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Determination of the binding mechanism of histone deacetylase inhibitors.

Authors :
Meyer-Almes FJ
Source :
Chemical biology & drug design [Chem Biol Drug Des] 2019 Jun; Vol. 93 (6), pp. 1214-1250. Date of Electronic Publication: 2018 Dec 30.
Publication Year :
2019

Abstract

This article places its focus on methods and tools enabling the elucidation of the mechanism by which ligands, small-molecule inhibitors, or substrates interact with zinc-containing bacterial or human members of the histone deacetylase family (HDACs). These methods include biochemical and biophysical approaches and can be subdivided into equilibrium and kinetic methods. More information about the exact mode of action can be obtained by combining these methods with specific mutant variants of the enzymes and/or series of structural similar ligands. All available equilibrium and kinetic data including additional information from 3D structures of HDAC-ligand complexes can be beneficially combined in a data analysis procedure called Integrated Global-Fit analysis eventually providing the most likely binding mechanism.<br /> (© 2018 John Wiley & Sons A/S.)

Details

Language :
English
ISSN :
1747-0285
Volume :
93
Issue :
6
Database :
MEDLINE
Journal :
Chemical biology & drug design
Publication Type :
Academic Journal
Accession number :
30480375
Full Text :
https://doi.org/10.1111/cbdd.13449