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Protective Effects of Apoptosis of Kupffer Cells Induced by Zoledronate Liposomes Following Hepatic Ischemia-Reperfusion Injury.
- Source :
-
Annals of transplantation [Ann Transplant] 2018 Nov 23; Vol. 23, pp. 815-821. Date of Electronic Publication: 2018 Nov 23. - Publication Year :
- 2018
-
Abstract
- BACKGROUND The goal of this study was to observe the effect of the apoptosis of Kupffer cells (KCs) selectively induced by zoledronate liposomes following the hepatic ischemia-reperfusion injury (IRI) in the rat liver transplantation model and to explore its mechanisms. MATERIAL AND METHODS The rat liver transplantation model was established using the improved Kamada method. Male Sprague Dawley rats were randomly divided into 3 groups: no liver transplantation or drug treatment (Group A); donor rats were injected with 1 mL normal saline through the tail vein for 3 continuous days before transplantation, and the donor liver was preserved in cold for 2 hours (Group B); donor rats were injected with 1 mL zoledronate liposomes (0.001 mg/mL) through the tail vein for 3 continuous days before transplantation, and the donor liver was preserved in cold for 2 hours (Group C). At 24 hours after transplantation, the receiving rats were sacrificed for sampling. RESULTS Compared with Group C and Group A, the bile secretion flow was dramatically decreased in Group B, whereas the serum liver function index [alanine aminotransferase (ALT), glutamate aminotransferase (AST), and γ-glutamyl transpeptidase (γ-GT)] was significantly increased (P<0.01), and the pathological injury area was obviously increased. Compared with Group B, the levels of serum interleukin1 (IL-1), tumor necrosis factor-α (TNF-α), and the apoptotic index in Group C were significantly decreased (P<0.05), and Suzuki scores of congestion, vacuolar degeneration, and necrosis were all reduced (P<0.05). CONCLUSIONS The apoptosis of KCs selectively induced by zoledronate liposomes inhibited the inflammatory cascade reaction induced by KC activation and reduced the release of cytokines and decreased the extent of IRI in the liver transplantation in animal model.
- Subjects :
- Animals
Biomarkers metabolism
Liposomes
Male
Protective Agents administration & dosage
Protective Agents therapeutic use
Random Allocation
Rats
Rats, Sprague-Dawley
Reperfusion Injury etiology
Reperfusion Injury metabolism
Reperfusion Injury pathology
Zoledronic Acid administration & dosage
Zoledronic Acid therapeutic use
Apoptosis drug effects
Kupffer Cells drug effects
Liver Transplantation
Protective Agents pharmacology
Reperfusion Injury prevention & control
Zoledronic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2329-0358
- Volume :
- 23
- Database :
- MEDLINE
- Journal :
- Annals of transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 30467306
- Full Text :
- https://doi.org/10.12659/AOT.909982