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Use of syringe filters to isolate Campylobacter species from stool samples.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2018 Dec; Vol. 155, pp. 78-81. Date of Electronic Publication: 2018 Nov 15. - Publication Year :
- 2018
-
Abstract
- We investigated the efficacy of syringe filters and membrane filters with different pore sizes for recovering Campylobacter. A syringe filter with a 0.45 μm pore size achieved the highest recovery rate (0.29%), while polycarbonate membrane filters with pore sizes of 0.6 μm and 0.4 μm recovered less Campylobacter, at 0.01% and 1.3 × 10 <superscript>-3</superscript> %, respectively. We also tested 601 diarrheic stool samples using membrane and syringe filtration methods. A total of 23 Campylobacter jejuni/coli were isolated from both syringe and membrane filtration; nine and one were isolated from only syringe or only membrane filtration, respectively (p < .05). The syringe filtration technique was better than membrane filtration for the isolation of Campylobacter.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Body Fluids microbiology
Campylobacter pathogenicity
Campylobacter Infections diagnosis
Campylobacter Infections microbiology
Campylobacter jejuni isolation & purification
Campylobacter jejuni pathogenicity
Diarrhea microbiology
Humans
Polycarboxylate Cement
Sensitivity and Specificity
Bacteriological Techniques methods
Campylobacter isolation & purification
Feces microbiology
Filtration instrumentation
Filtration methods
Syringes
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 155
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 30448215
- Full Text :
- https://doi.org/10.1016/j.mimet.2018.11.012