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Use of syringe filters to isolate Campylobacter species from stool samples.

Authors :
Hou S
Wu X
Zhou Y
He P
Chen S
Source :
Journal of microbiological methods [J Microbiol Methods] 2018 Dec; Vol. 155, pp. 78-81. Date of Electronic Publication: 2018 Nov 15.
Publication Year :
2018

Abstract

We investigated the efficacy of syringe filters and membrane filters with different pore sizes for recovering Campylobacter. A syringe filter with a 0.45 μm pore size achieved the highest recovery rate (0.29%), while polycarbonate membrane filters with pore sizes of 0.6 μm and 0.4 μm recovered less Campylobacter, at 0.01% and 1.3 × 10 <superscript>-3</superscript> %, respectively. We also tested 601 diarrheic stool samples using membrane and syringe filtration methods. A total of 23 Campylobacter jejuni/coli were isolated from both syringe and membrane filtration; nine and one were isolated from only syringe or only membrane filtration, respectively (p < .05). The syringe filtration technique was better than membrane filtration for the isolation of Campylobacter.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1872-8359
Volume :
155
Database :
MEDLINE
Journal :
Journal of microbiological methods
Publication Type :
Academic Journal
Accession number :
30448215
Full Text :
https://doi.org/10.1016/j.mimet.2018.11.012