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Studies on the effects of microencapsulated human mesenchymal stem cells in RGD-modified alginate on cardiomyocytes under oxidative stress conditions using in vitro biomimetic co-culture system.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2019 Feb 15; Vol. 123, pp. 512-520. Date of Electronic Publication: 2018 Nov 13. - Publication Year :
- 2019
-
Abstract
- Stem cell therapy has been recognized as a promising approach for myocardium regeneration post myocardial infarction (MI); however, it unfortunately often remains a challenge because of poor survival of transplanted cells and a lack of clear understanding of their interactions with host cells. High oxidative stress at heart tissues post MI is considered one of the important factors damaging transplanted cells and native cells/tissues. Here, we employed an in vitro co-culture system, capable of mimicking cases of stem cell transplantation into the myocardium presenting high oxidative stress, using human mesenchymal stem cells (hMSCs) encapsulated in alginate or cell interactive Arg-Gly-Asp (RGD) peptide-modified alginate micro-hydrogels. Under H <subscript>2</subscript> O <subscript>2</subscript> -induced oxidative stress conditions, viabilities of hMSCs and CMs were significantly higher in their co-culture than in their individual monolayer cultures. Expression of cardiac muscle markers remained high even with H <subscript>2</subscript> O <subscript>2</subscript> treatment when cardiomyocytes (CMs) were co-cultured with hMSCs in RGD-alginate. Higher levels of various growth factors (associated with angiogenesis, cardiac regeneration, and contractility) were found in co-culture (noticeably with RGD-alginate) compared to monolayer cultures of CMs or hMSCs. These results can benefit the study of in vivo MI progression with transplanted stem cells and the development of effective stem cell-based therapeutic strategies for various oxidative stress-related diseases.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Cell Line
Cells, Immobilized cytology
Coculture Techniques
Humans
Mesenchymal Stem Cells cytology
Myocardium cytology
Myocardium metabolism
Myocytes, Cardiac cytology
Alginates chemistry
Cells, Immobilized metabolism
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells metabolism
Myocytes, Cardiac metabolism
Oligopeptides chemistry
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 123
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 30445088
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.11.115