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Allorhizocola rhizosphaerae gen. nov., sp. nov., a new member of Micromonosporaceae isolated from rhizosphere soil of the plant Calligonum mongolicum.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2019 Jan; Vol. 69 (1), pp. 109-115. Date of Electronic Publication: 2018 Nov 16. - Publication Year :
- 2019
-
Abstract
- The taxonomic position of an actinobacterium, designated CPCC 204380 <superscript>T</superscript> , which was isolated from a rhizosphere soil sample of the plant Calligonum mongolicum collected from Xinjiang Province, China, was established using a polyphasic approach. Vegetative hyphae developed well and globose bodies formed from aged hyphae. Spore chains that differentiated from the vegetative hyphae contained non-motile rod-shaped spores. The peptidoglycan contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid as the diagnostic amino acids. The acyl type of the peptidoglycan was glycolyl. Glucose, mannose, ribose and xylose were detected in whole-cell hydrolysates. The predominant menaquinone was MK-10(H8), followed by MK-10(H6) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and C17 : 1ω9c. The genomic G+C content was 64.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204380 <superscript>T</superscript> should be placed in the family Micromonosporaceae, in which it formed a distinct lineage next to the genera Rhizocola, Catellatospora, Catelliglobosispora, Hamadaea and Allocatelliglobosispora. It shared the highest 16S rRNA gene sequence similarities with Rhizocola hellebori K12-0602 <superscript>T</superscript> (96.1 %), Catellatospora chokoriensis 2-25/1 <superscript>T</superscript> (95.9 %), Catelliglobosispora koreensis DSM 44566 <superscript>T</superscript> (95.9 %), Hamadaea tsunoensis DSM 44101 <superscript>T</superscript> (95.3 %) and Allocatelliglobosispora scoriae Sco-B14 <superscript>T</superscript> (94.2 %), and less than 94.0 % sequence similarity with other validly described species. The combination of phylogenetic analysis and phenotypic characteristics supported the proposal of strain CPCC 204380 <superscript>T</superscript> as representing a novel species of a new genus in the family Micromonosporaceae, for which the name Allorhizocola rhizosphaerae gen. nov., sp. nov. is proposed. CPCC 204380 <superscript>T</superscript> (=DSM 102292 <superscript>T</superscript> =KCTC 39746 <superscript>T</superscript> ) is the type strain of the type species.
- Subjects :
- Bacterial Typing Techniques
Base Composition
Cell Wall chemistry
China
DNA, Bacterial genetics
Diaminopimelic Acid chemistry
Fatty Acids chemistry
Micromonosporaceae isolation & purification
Peptidoglycan chemistry
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Vitamin K 2 chemistry
Micromonosporaceae classification
Phylogeny
Polygonaceae microbiology
Rhizosphere
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 69
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30444473
- Full Text :
- https://doi.org/10.1099/ijsem.0.003114