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Comparison of multi-wall carbon nanotube and nitrogen-doped multi-wall carbon nanotube effects on lung function and airway reactivity in rats.
- Source :
-
Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2019 Feb 01; Vol. 364, pp. 153-163. Date of Electronic Publication: 2018 Nov 10. - Publication Year :
- 2019
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Abstract
- Incorporation of multi-wall carbon nanotubes (MWCNT) into materials has raised concerns about their potential hazards to manufacturing workers. In animal models, airway inflammation and lung fibrosis follow aspiration, instillation, and inhalation exposures to MWCNT. However, the effects of MWCNT on pulmonary function, airway reactivity and airway epithelium function following inhalation exposure has not been studied. We investigated whether inhaled MWCNT affects lung resistance (R <subscript>L</subscript> ) and dynamic compliance (C <subscript>dyn</subscript> ), reactivity to inhaled methacholine (MCh), epithelial regulation of airway reactivity to MCh in vitro, and airway epithelial ion transport. Male rats were exposed by whole body inhalation for 6 h to air or aerosolized MWCNT (0.5, 1 or 5 mg/m <superscript>3</superscript> ) for one or nine days. Eighteen h after 1 d exposure to 5 mg/m <superscript>3</superscript> MWCNT, basal R <subscript>L</subscript> was increased and basal C <subscript>dyn</subscript> was decreased; changes did not persist for 7 d. Reactivity to MCh (R <subscript>L</subscript> ) was increased and C <subscript>dyn</subscript> responses were decreased at 18 h, but not 7 d after exposure to 1 and 5 mg/m <superscript>3</superscript> MWCNT. The effects of i.t.-instilled MWCNT and nitrogen-doped MWCNT (N-MWCNT) on pulmonary function and reactivity to MCh at doses comparable to deposition after inhalation of 5 mg/m <superscript>3</superscript> at 1 d and 0.5, 1, and 5 mg/m <superscript>3</superscript> MWCNT 9 d-exposures were compared. Both nanoparticles increased airway reactivity (R <subscript>L</subscript> ); N-MWCNT did not affect C <subscript>dyn</subscript> responses. Lung function and airway reactivity are altered following a single MWCNT inhalation and generally subside over time. Given i.t., MWCNT's and N-MWCNT's effects were comparable, but N-MWCNT evoke smaller changes in C <subscript>dyn</subscript> responses.<br /> (Published by Elsevier Inc.)
- Subjects :
- Aerosols
Airway Resistance drug effects
Animals
Bronchial Hyperreactivity metabolism
Bronchial Hyperreactivity physiopathology
Bronchial Provocation Tests
Bronchoconstrictor Agents administration & dosage
Dose-Response Relationship, Drug
Epithelial Cells drug effects
Epithelial Cells metabolism
Inhalation Exposure
Ion Transport
Lung metabolism
Lung physiopathology
Lung Compliance drug effects
Male
Methacholine Chloride administration & dosage
Nanotubes, Carbon chemistry
Nitrogen chemistry
Permeability
Rats, Sprague-Dawley
Risk Assessment
Time Factors
Bronchial Hyperreactivity chemically induced
Bronchoconstriction drug effects
Lung drug effects
Nanotubes, Carbon toxicity
Nitrogen toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0333
- Volume :
- 364
- Database :
- MEDLINE
- Journal :
- Toxicology and applied pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 30423287
- Full Text :
- https://doi.org/10.1016/j.taap.2018.11.004