Back to Search
Start Over
Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets.
- Source :
-
The Korean journal of parasitology [Korean J Parasitol] 2018 Oct; Vol. 56 (5), pp. 437-446. Date of Electronic Publication: 2018 Oct 31. - Publication Year :
- 2018
-
Abstract
- To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
- Subjects :
- Animals
Biomarkers analysis
Prevalence
Republic of Korea epidemiology
Swine
Swine Diseases diagnosis
Toxoplasmosis, Animal diagnosis
Antibodies, Protozoan analysis
Enzyme-Linked Immunosorbent Assay methods
Food Contamination analysis
Meat analysis
Swine Diseases epidemiology
Swine Diseases parasitology
Toxoplasma immunology
Toxoplasmosis, Animal epidemiology
Toxoplasmosis, Animal parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1738-0006
- Volume :
- 56
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Korean journal of parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 30419729
- Full Text :
- https://doi.org/10.3347/kjp.2018.56.5.437