Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells.

Authors :: Périchon B
Guignot J
Szili N
Gao C
Poyart C
Trieu-Cuot P
Dramsi S
Source :: Microbes and infection [Microbes Infect] 2019 Mar; Vol. 21 (2), pp. 99-103. Date of Electronic Publication: 2018 Nov 10.
Publication Year :: 2019
Abstract: The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages.<br /> (Copyright © 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)

Subjects :: Adhesins, Bacterial genetics
Cell Wall metabolism
Endothelial Cells microbiology
Humans
Macrophages microbiology
Phagocytosis
Streptococcus agalactiae pathogenicity
Streptococcus agalactiae physiology
Adhesins, Bacterial metabolism
Bacterial Adhesion
Fimbriae, Bacterial metabolism
Operon genetics
Streptococcal Infections microbiology
Streptococcus agalactiae genetics

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