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Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs).

Authors :
Cottrell JA
Burgess D
Michaels S
Rogers MB
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1891, pp. 1-8.
Publication Year :
2019

Abstract

Many research methods exist to elucidate the functions of BMPs during osteogenesis. This chapter briefly reviews common immortalized mesenchymal cell types used to measure the efficacy of osteogenic factors like BMP-2. Detailed information regarding media and culture conditions are provided. Parameters relevant to experimental reproducibility and cell line authentication are discussed.

Subjects

Subjects :
Animals
Bone Morphogenetic Protein 2 genetics
Bone Morphogenetic Protein 2 metabolism
Bone Morphogenetic Proteins metabolism
Cell Differentiation genetics
Cell Line
Cells, Cultured
Humans
Osteoblasts cytology
Osteoblasts metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Transforming Growth Factor beta genetics
Transforming Growth Factor beta metabolism
Bone Morphogenetic Proteins genetics

Details

Language :
English
ISSN :
1940-6029
Volume :
1891
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
30414121
Full Text :
https://doi.org/10.1007/978-1-4939-8904-1_1
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