Back to Search
Start Over
NME proteins regulate class switch recombination.
- Source :
-
FEBS letters [FEBS Lett] 2019 Jan; Vol. 593 (1), pp. 80-87. Date of Electronic Publication: 2018 Nov 23. - Publication Year :
- 2019
-
Abstract
- Class switch recombination (CSR) in B cells involves deletion-recombination at switch (S) region DNA and is important for the diversification of antibody isotypes during an immune response. Here, we identify two NME [NM23/NDPK (nucleoside diphosphate kinase)] isoforms, NME1 and NME2, as novel players in this process. Knockdown of NME2 leads to decreased CSR, while knockdown of the highly homologous NME1 results in increased CSR. Interestingly, these NME proteins also display differential occupancy at S regions during CSR despite their homology; NME1 binds to S regions prior to stimulation, while NME2 binds to S regions only after stimulation. To the best of our knowledge, this represents the first report of a role for these proteins in the regulation of CSR.<br /> (© 2018 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Subjects :
- Animals
Cell Proliferation
Cells, Cultured
Gene Knockdown Techniques
Immunoglobulin Class Switching
Immunoglobulin Heavy Chains metabolism
Immunoglobulin Switch Region
Mice
NM23 Nucleoside Diphosphate Kinases genetics
Protein Binding
B-Lymphocytes metabolism
Immunoglobulin Heavy Chains chemistry
NM23 Nucleoside Diphosphate Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 593
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 30411342
- Full Text :
- https://doi.org/10.1002/1873-3468.13290