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A Screen Using iPSC-Derived Hepatocytes Reveals NAD + as a Potential Treatment for mtDNA Depletion Syndrome.

Authors :
Jing R
Corbett JL
Cai J
Beeson GC
Beeson CC
Chan SS
Dimmock DP
Lazcares L
Geurts AM
Lemasters JJ
Duncan SA
Source :
Cell reports [Cell Rep] 2018 Nov 06; Vol. 25 (6), pp. 1469-1484.e5.
Publication Year :
2018

Abstract

Patients with mtDNA depletion syndrome 3 (MTDPS3) often die as children from liver failure caused by severe reduction in mtDNA content. The identification of treatments has been impeded by an inability to culture and manipulate MTDPS3 primary hepatocytes. Here we generated DGUOK-deficient hepatocyte-like cells using induced pluripotent stem cells (iPSCs) and used them to identify drugs that could improve mitochondrial ATP production and mitochondrial function. Nicotinamide adenine dinucleotide (NAD) was found to improve mitochondrial function in DGUOK-deficient hepatocyte-like cells by activating the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α). NAD treatment also improved ATP production in MTDPS3-null rats and in hepatocyte-like cells that were deficient in ribonucleoside-diphosphate reductase subunit M2B (RRM2B), suggesting that it could be broadly effective. Our studies reveal that DGUOK-deficient iPSC-derived hepatocytes recapitulate the pathophysiology of MTDPS3 in culture and can be used to identify therapeutics for mtDNA depletion syndromes.<br /> (Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
2211-1247
Volume :
25
Issue :
6
Database :
MEDLINE
Journal :
Cell reports
Publication Type :
Academic Journal
Accession number :
30404003
Full Text :
https://doi.org/10.1016/j.celrep.2018.10.036