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Screening and evaluation of antiviral compounds against Equid alpha-herpesviruses using an impedance-based cellular assay.
- Source :
-
Virology [Virology] 2019 Jan 02; Vol. 526, pp. 105-116. Date of Electronic Publication: 2018 Oct 26. - Publication Year :
- 2019
-
Abstract
- Equid alpha-herpesviruses (EHV) are responsible for different diseases in equine population. EHV-1 causes respiratory diseases, abortions and nervous disorders, EHV-4 causes respiratory diseases and sporadic abortion, while EHV-3 is responsible of equine coital exanthema. In view of the lack of efficacy of vaccines against EHV-1 and EHV-4 and in the absence of vaccines against EHV-3, the use of antiviral treatment is of great interest. In this study, we documented the interest of the Real-Time Cell Analysis (RTCA) technology to monitor the cytopathic effects induced by these viruses on equine dermal cells, and established the efficacy of this method to evaluate the antiviral effect of aciclovir (ACV) and ganciclovir (GCV). In addition, the RTCA technology has also been found appropriate for the high-throughput screening of small molecules against EHV, allowing the identification of spironolactone as a novel antiviral against EHV.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cytopathogenic Effect, Viral drug effects
Herpesviridae Infections pathology
Herpesviridae Infections virology
Herpesvirus 1, Equid classification
Herpesvirus 3, Equid drug effects
Herpesvirus 4, Equid drug effects
Horses
Spironolactone pharmacology
Antiviral Agents pharmacology
Electric Impedance
Herpesviridae Infections veterinary
Herpesvirus 1, Equid drug effects
High-Throughput Screening Assays methods
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 526
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 30388626
- Full Text :
- https://doi.org/10.1016/j.virol.2018.10.013