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Deletion of the epigenetic regulator GcnE in Aspergillus niger FGSC A1279 activates the production of multiple polyketide metabolites.
- Source :
-
Microbiological research [Microbiol Res] 2018 Dec; Vol. 217, pp. 101-107. Date of Electronic Publication: 2018 Oct 15. - Publication Year :
- 2018
-
Abstract
- Epigenetic modification is an important regulatory mechanism in the biosynthesis of secondary metabolites in Aspergillus species, which have been considered to be the treasure trove of new bioactive secondary metabolites. In this study, we reported that deletion of the epigenetic regulator gcnE, a histone acetyltransferase in the SAGA/ADA complex, resulted in the production of 12 polyketide secondary metabolites in A. niger FGSC A1279, which was previously not known to produce toxins or secondary metabolites. Chemical workup and structural elucidation by 1D/2D NMR and high resolution electrospray ionization mass (HR-ESIMS) yielded the novel compound nigerpyrone (1) and five known compounds: carbonarone A (2), pestalamide A (3), funalenone (4), aurasperone E (5), and aurasperone A (6). Based on chemical information and the literature, the biosynthetic gene clusters of funalenone (4), aurasperone E (5), and aurasperone A (6) were located on chromosomes of A. niger FGSC A1279. This study found that inactivation of GcnE activated the production of secondary metabolites in A. niger. The biosynthetic pathway for nigerpyrone and its derivatives was identified and characterized via gene knockout and complementation experiments. A biosynthetic model of this group of pyran-based fungal metabolites was proposed.<br /> (Crown Copyright © 2018. Published by Elsevier GmbH. All rights reserved.)
- Subjects :
- Base Sequence
Biosynthetic Pathways genetics
Chromones metabolism
Diketopiperazines metabolism
Fungal Proteins genetics
Gene Expression Regulation, Fungal
Gene Knockout Techniques
Histone Acetyltransferases genetics
Homologous Recombination
Indole Alkaloids metabolism
Ketones metabolism
Multigene Family
Polycyclic Compounds metabolism
Pyrones metabolism
Secondary Metabolism genetics
Aspergillus niger genetics
Aspergillus niger metabolism
Epigenomics
Genes, Fungal genetics
Polyketides metabolism
Sequence Deletion
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0623
- Volume :
- 217
- Database :
- MEDLINE
- Journal :
- Microbiological research
- Publication Type :
- Academic Journal
- Accession number :
- 30384904
- Full Text :
- https://doi.org/10.1016/j.micres.2018.10.004