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Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.
- Source :
-
Nature methods [Nat Methods] 2018 Nov; Vol. 15 (11), pp. 936-939. Date of Electronic Publication: 2018 Oct 30. - Publication Year :
- 2018
-
Abstract
- Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.
- Subjects :
- Animals
Color
Female
Ferrets
Fluorescent Dyes
Glutamic Acid analysis
Male
Mice, Inbred C57BL
Neurons metabolism
Retina metabolism
Visual Cortex metabolism
Glutamic Acid metabolism
Green Fluorescent Proteins metabolism
Microscopy, Fluorescence methods
Neurons cytology
Retina cytology
Visual Cortex cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 15
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 30377363
- Full Text :
- https://doi.org/10.1038/s41592-018-0171-3